The catalogue contains molecular data (including polymorphisms and haplotypes) together with detailed information on sampling, taxonomy and geographical origin of the most important European marine fish species.
FishTrace provides information for the molecular identification of target species based on the sequences of the mitochondrial cytochrome b gene and the nuclear rhodopsin gene . This molecular data forms the basis of several key objectives: validation strategy, genetic variation in widespread species, practical tools for species differential diagnosis, elaboration of biological reference collections, and genetic catalogue of marine fishes.
The species exhibiting wide distribution covering several of the geographical sea areas sampled, allow for the detection of sequence variation in the genes analysed. An objective of FishTrace network is to use the genetic data collected for the identification populations-specific sequence. This information compiled in the FishTrace database provides particular genotypic marks of the species of wide distribution in Europe.
Contents of the catalogue:
- Species, genus and bibliography information
- Specimens analysed in FishTrace, their biogeography with GIS representation, their DNA analysis
- Nucleotide sequence of a complete mitochondrial gene (cytochrome b: 1141 bp) and part of a nuclear gene (rhodopsin: 460 bp) from each targeted fish species
- Extracted reference material and tissues and their actual location
- Methodologies used (to extract the DNA and to amplify by PCR)
- Information about polymorphisms detected